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spoIVFB [2020-06-11 09:15:14]
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spoIVFB [2020-06-11 09:15:14]

intramembrane metalloprotease (site 2 protease), processing of pro-sigma-K to active [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]
locus
BSU_27970
pI
8.48
mw
33.49 kDa
protein length
288 aa Sequence Blast
gene length
867 bp Sequence Blast
function
processing of pro-sigma-K to active [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]
product
intramembrane metalloprotease
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.3|Proteolysis during sporulation/ germination]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,855,973 2,856,839

    The protein

    Catalyzed reaction/ biological activity

  • processing of pro-sigma-K to active [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK] [Pubmed|19805276]

    • Protein family

  • [SW|peptidase M50B family] (according to UniProt)

    • [SW|Domains]

  • C-terminal [SW|CBS domain], this domain binds ATP [Pubmed|19805276]

    • Effectors of protein activity

  • ATP regulates substrate access to the active site and renders cleavage sensitive to the cellular energy level [Pubmed|19805276]
  • [protein|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|SpoIVFB] is kept inactive by the interaction with [protein|AB2A3422277040107AAF90358BBE58608F8E0738|SpoIVFA] and [protein|CB5D3A2E5BEE336539BEB2D7C5D9C3A63C78FD01|BofA] [pubmed|15087499]

    • [SW|Localization]

  • mother cell integral membrane protein [Pubmed|24243021,11959848]

    • Expression and Regulation

    Operons

    genes
    [gene|AB2A3422277040107AAF90358BBE58608F8E0738|spoIVFA]-[gene|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB]
    description
    [Pubmed|1942049]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,1942049], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

    • regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|15383836], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]

    • regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE], [SW|SpoIIID]) [Pubmed|15699190,1942049,15383836]

    • additional information

  • the domains that inhibits [protein|search|SpoIVFB] is cleaved off by [protein|search|CtpB] (second cleavage) [PubMed|24243021]
    • view in new tab

    genes
    [gene|AB2A3422277040107AAF90358BBE58608F8E0738|spoIVFA]-[gene|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB]-[gene|11927EB0448839D047EC7BF70EC65156DAB481D3|rplU]-[gene|B27548A4BBF396C9BA102A80ACB08B21262CEA30|prp]-[gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA]
    description
    [Pubmed|22383849]

    regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE], [SW|SpoIIID]) [Pubmed|15699190,1942049,15383836]

    • additional information

  • the domains that inhibits [protein|search|SpoIVFB] is cleaved off by [protein|search|CtpB] (second cleavage) [PubMed|24243021]
    • view in new tab

    Biological materials

    Mutant

  • BKE27970 ([gene|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATCTTTAAGATAAGGTCGA, downstream forward: _UP4_TAAAACTGATTGACAAACGC
  • BKK27970 ([gene|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATCTTTAAGATAAGGTCGA, downstream forward: _UP4_TAAAACTGATTGACAAACGC

    • References

    Reviews

  • 20836086,23479438,19189971,24099006,31350897

    • Original Publications

  • 11959848,9501233,12940997,1577688,12060714,9078383,1942049,10611287,15383836,16818230,19805276,15699190,23585539,23995631,15087499,24243021,22383849,26953342,29180425,30403663